Scale-up of a cell culture with Nucleo single-use bioreactor
Production of recombinant proteins with a CHO-based cell line using the Nucleo-200 disposable bioreactor is compared to production with a classical stainless steel bioreactor
Run 1: The tests first involved comparison of culture results at a 20L working volume with those obtained at a scale of 80L, respectly performed with a New Brunswick Scientific stainless steel bioreactor and the Nucleo-200 disposable bioreactor.
The next objective was to study the performance of the Nucleo disposable bioreactor with a working volume taken up to 180L.
Run 2: In certain applications, the perfusion cell culture proves to have various benefits compared to traditional methods. Moreover, the type of process equipment also significantly affects efficiency. In an attempt to optimize performance, the Nucleo-200 disposable bioreactor created by ATMI® LifeSciences and Pierre Guerin Technologies® represents an interesting alternative. Consequently, tests were carried out to assess the reaction of the Nucleo-200 disposable bioreactor, particularly with a minimum volume, at low and high densities in batch and perfusion mode.
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stainless steel storage food vessels - stainless steel preparation vessels - stainless steel pharmaceutical vessels - stainless steel cheese vats - stainless steel wine making tanks - stainless steel starter vessels - stainless steel dairy vessels - stainless steel food process vessels - stainless steel vinification tanks - cleaning in place units - agitators for stainless steel vessels - stainless steel cookers mixers - cosmetic cream mixers - cosmetic industrial emulsifiers - centrifugal pumps for food and pharmaceutical industry - stainless steel maturation tanks - stainless steel cristallisation tanks
|Legals mentions - Design and production|